Investigation of the Doughnut Effect in Cy3-Labeled Protein Microarrays using Scanning Near-Field Optical Microscope

We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known “doughnut effect” observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image.     

等离子体中电磁波的粒子模拟

本文介绍了1 2/2维相对论性、电磁模等离子体粒子模拟的方法和原理。系统地以计算机模拟了由电子气体组成的等离子体中的各种电磁波。在无外加静磁场、加外横磁场、加外纵向磁场等不同情况下,分别得到静电波、电磁波、寻常波、非寻常波、ω=nω_c的磁回旋共振、左旋波、右旋波等。其色散关系、截止频率、共振频率、极限相速度均与理论结果一致。     

纳米晶Y_2SiO_5∶Eu的浓度猝灭研究

报道了分别用溶胶－凝胶法合成的Ｙ２ＳｉＯ５∶Ｅｕ纳米晶和用高温固相法合成常规尺度的Ｙ２ＳｉＯ５∶Ｅｕ材料的光致发光光谱和猝灭浓度的实验研究．结果表明：纳米Ｙ２－ｘＥｕｘＳｉＯ５比常规尺度的Ｙ２－ｘＥｕｘＳｉＯ５有更高的猝灭浓度和更高的发光亮度．理论分析认为这是由于在纳米材料中能量共振传递被阻断和猝灭中心在各个纳米晶内分布的涨落造成的．这个结果为高亮度的Ｙ２ＳｉＯ５∶Ｅｕ纳米材料的实际开发应用展示了广阔前景．     

一类多维的非线性Schrdinger方程组的初边值问题

本文考虑一类具有耗散与磁场效应的多维非线性Schrdinger型方程组的初边值问题。使用积分估计(包括L~p—L~q估计)证明了整体解的存在性。     

A text-to-speech system with high intelligibility and naturalness for Chinese

I.IntroductionResearchesonChinesesynthesisdisclosethatonlywhenboththesegmentalandsupraseg-melltalfeaturesofthesyntheticspeecharesimilartothoseofthellaturalone,thesyntheticspeechwillsoundintelligibleandnatural[1].Amongekistingsynthetictechniques,theapproachbasedonacousticparametersca-nadustboththesegmentalandsuprasegmentalfeaturesofsyntheticunitsfiekiblyandcanbeconsideredasthemostreasonablesynthetictechniqueintheory.However,theparameterbasedsynthesizerisoverAfependentonthedevelopmentsofparamet…     

利用谱线宽度测人发中的微量元素

通过实验证明了可以利用谱线宽度做永久工作曲线用于光谱定量分析。给出了用于测量人发中微量元素Ｃｕ，Ｚｎ，Ｆｅ和Ｃａ的永久工作曲线，利用这些工作曲线对实际样品进行了测定，所得结果与用ＩＣＰ－ＡＥＳ测得的结果相吻合。     

On a class of maximally hypoelliptic operators

The maximal hypoellipticity of the operator X ₁ ^2m , ... X _k ^2m is proved, where X_j are first-order pseudodifferential operators and m is a natural number. It is assumed that the set of these operators and their first-order commutators is an elliptic system. The results obtained generalize the well-known results of Hormander, Oleinik, and Radkevich, Kohn, Helffer, and Nourrigat, and Rothshield and Stein. Bibliography: 9 titles. Translated from Trudy Seminara imeni I. G. Petrovskogo, No. 17, pp. 3–26, 1994.     

A Novel Method to Synthesize Polyorganozircosilazane

A new kind of polyorganozircosilazane as Si/Zr/C/N-based ceramic precursor was synthesized from the condensation reaction of hexamethyleyclotrisilazane lithium salts(D3^NLi）and zirconium tetrachloride(ZrCl4).In the presence of N,N,N’,N’-tetramethyl ethylene diamine(TMEDA),polyorganozircosilazane was obtained in high yield.     

Digisorb 2600型物理吸附仪真空系统检漏、故障分析及对策

就Ｄｉｇｉｓｏｒｂ２６００型物理吸附仪的真空系统拟出了表格形式的基本检漏步骤，并在此基础上详细介绍了几例特殊真空故障的查找，分析及解决方法。